hcdsim pbam
hcdsim pbam command to process cell bams, including sort, markduplications and add reads group, using samtools tools.
usage: hcdsim pbam [-h] [-r] [-o] [-g] [-b] [-gv] [-cno] [-eno] [-t] [--random_seed] [--samtools]
options:
-h, --help show this help message and exit
-r , --ref_genome Path to reference genome [required]
-o , --outdir Output directory (default: current directory)
-g , --ignore Path to the exclusion list of contigs file (default: none)
-b , --bin_size The fixed bin size, with or without "kb" or "Mb" (default: 100kb)
-gv , --genome_version
Genome version: hg19 or hg38 (default: hg38)
-cno , --clone_no The clone number contained in evolution tree, including normal clone (default: 2)
-eno , --cell_no The total cell number for this simulation dataset (default: 2)
-t , --thread Number of parallel jobs to use (default: equal to number of available processors)
--random_seed Random seed for reproducibility (default: none)
--samtools Path to the executable "samtools" file (default: in $PATH)